CSIRO Division of Plant Industry, GPO Box 1600, 2601, Canberra, ACT, Australia.
Theor Appl Genet. 1992 Sep;84(7-8):845-50. doi: 10.1007/BF00227395.
Random cDNA sequences synthesized from poly A(+) RNA extracted from germinated urediospores of the flax rust fungus, Melampsora lini, were used as probes to detect restriction fragment length polymorphisms (RFLPs) in three races of M. lini originating from cultivated flax, Linum usitatissimum, and one race originating from Australian native flax, L. marginale. Fourteen out of 22 probes tested detected RFLPs in the three races from cultivated flax while 19 of the probes detected polymorphisms between these three races and the race from L. marginale. The segregation of seven RFLPs was determined in a family of 19 F2 progeny derived from a cross between two of the rust races. With six of these the inheritance was consistent, in each case, with the segregation of alleles at a single locus. Inheritance of the seventh was unusual and an explanation involving two loci with null alleles at each was proposed. No linkage was detected between any of the RFLP loci and nine unlinked loci specifying avirulence.
从萌发的亚麻锈菌(Urediospores)的多 A(+) RNA 中提取的 cDNA 序列被用作探针，以检测来自栽培亚麻（Linum usitatissimum）的三种亚麻锈菌（Melampsora lini）和一种源自澳大利亚本地亚麻（L. marginale）的锈菌的限制片段长度多态性（RFLP）。在测试的 22 个探针中，有 14 个探针在来自栽培亚麻的三个锈菌中检测到 RFLP，而 19 个探针在这三个锈菌和来自 L. marginale 的锈菌之间检测到多态性。在来自两个锈菌杂交的 19 个 F2 后代的一个家系中，确定了 7 个 RFLP 的分离。其中 6 个的遗传与一个基因座上等位基因的分离一致。第七个的遗传是不寻常的，提出了涉及每个基因座上的两个具有无效等位基因的解释。在任何 RFLP 基因座和 9 个不连锁的无毒基因座之间都没有检测到连锁。
