National Chemical Laboratory, Plant Molecular Biology Unit, 411 008, Pune, India.
Theor Appl Genet. 1995 Nov;91(6-7):856-61. doi: 10.1007/BF00223892.
Genetic variability in six host genotype-specific pathotypes of pearl millet downy mildew pathogen S. graminicola was studied at the molecular level using mini- and micro-satellites. Our results indicated that microsatellites (GAA)6, (GACA)4, and especially (GATA)4 were quite informative and showed high levels of polymorphism among the pathotypes. The six pathotypes could be classified into five groups based on the cluster analysis of their genetic similarities, thereby confirming the existence of distinct host genotype-specific virulence in S. graminicola pathotypes. We demonstrate, for the first time, the use of DNA fingerprinting to detect genetic variation in downy mildew fungus of pearl millet.
利用微卫星和小卫星技术，在分子水平上研究了 6 种谷子霜霉病菌宿主基因型专化型的遗传变异性。研究结果表明，微卫星（GAA）6、（GACA）4 以及（GATA）4 非常具有信息量，并且在不同的专化型中表现出高水平的多态性。根据遗传相似性聚类分析，这 6 种专化型可以分为 5 组，从而证实了谷子霜霉病菌专化型中存在明显的宿主基因型专化性毒力。我们首次证明，利用 DNA 指纹图谱可以检测谷子霜霉菌的遗传变异。
