Experimental Farm, Kobe University, 1348 Uzurano, Kasai, 675-21, Hyogo, Japan.
Theor Appl Genet. 1992 Sep;84(7-8):851-8. doi: 10.1007/BF00227396.
Intraspecific variation of a wild potato species, Solanum acaule Bitt., was analyzed by RFLPs of genomic DNA. One hundred and five accessions were selected throughout the distribution area, including all subspecies, i.e., ssp. albicans (hexaploid), ssp. punae (tetraploid), ssp. acaule (tetraploid) and ssp. aemulans (tetraploid). Twenty-seven low-copy DNA clones (probes) were Southern hybridized with EcoRI, EcoRV, HindIII, and XbaI digests of total DNA of all accessions. In total, 238 RFLPs were detected from 94 enzyme x probe combinations. Among them, 49 RFLPs were specific to ssp. albicans, suggesting that the additional third genome is distinct from its two other genomes. RFLPs between and within subspecies were analyzed by principal component analysis. DNA similarities between subspecies coincided with a former taxonomic treatment in the sense that ssp. albicans is the most distantly related to ssp. acaule and ssp. aemulans is distantly related. Subspecies acaule and ssp. punae were indistinguishable. In addition, RFLPs could be used to distinguish groups within subspecies. Subspecies aemulans, confined to Argentina, was divided into two populations, one from the province of La Rioja and the other from the province of Jujuy. In ssp. acaule, some accessions from the southernmost distribution area were clearly distinguishable, while the others varied continuously, showing a geographical cline from Peru to Argentina.
对野生马铃薯种 Solanum acaule Bitt. 的种内变异进行了分析，方法是用基因组 DNA 的 RFLPs。在整个分布区选择了 105 个个体，包括所有亚种，即 ssp. albicans（六倍体）、ssp. punae（四倍体）、ssp. acaule（四倍体）和 ssp. aemulans（四倍体）。用 EcoRI、EcoRV、HindIII 和 XbaI 消化所有个体的总 DNA 后，Southern 杂交了 27 个低拷贝 DNA 克隆（探针）。从 94 个酶 x 探针组合中总共检测到 238 个 RFLPs。其中，49 个 RFLPs 是 ssp. albicans 特有的，这表明第三个额外基因组与其他两个基因组明显不同。通过主成分分析分析了亚种间和亚种内的 RFLPs。亚种间的 DNA 相似性与以前的分类处理一致，即 ssp. albicans 与 ssp. acaule 的亲缘关系最远，ssp. aemulans 的亲缘关系次之。亚种 acaule 和 ssp. punae 无法区分。此外，RFLPs 可用于区分亚种内的群体。局限于阿根廷的亚种 aemulans 分为两个群体，一个来自拉里奥哈省，另一个来自胡胡伊省。在 ssp. acaule 中，来自最南端分布区的一些个体明显可区分，而其他个体则连续变化，从秘鲁到阿根廷呈地理渐变。
