Vegetable Crops Research Unit, USDA, Agricultural Research Service, Department of Horticulture, University of Wisconsin, 1575 Linden Drive, 53706-1590, Madison, WI, USA.
Theor Appl Genet. 1996 Apr;92(5):532-40. doi: 10.1007/BF00224555.
We evaluated chloroplast DNA (cpDNA), isozymes, single to low-copy nuclear DNA (RFLPs), and random amplified polymorphic DNAs (RAPDs) in terms of concordance for genetic distance of 15 accessions each of Solanum etuberosum and S. palustre, and 4 accessions of S. fernandezianum. These self-compatible, diploid (2n=24), and morphologically very similar taxa constitute all species in Solanum sect. Etuberosum, a group of non-tuber-bearing species closely related to Solanum sect. Petota (the potato and its wild relatives). Genetic distance and multidimentional scaling results show general concordance of isozymes, RFLPs and RAPDs between all three taxa; cpDNA shows S. etuberosum and S. palustre to be more similar to each other than to S. fernandezianum. Interspecific sampling variance shows a gradation of resolution from allozyme (low) to RAPD to RFLP (high); while intraspecific comparisons graded from RFLPs (low) to RAPDs (high; lack of sufficient allozyme variability within species precluded comparisons for allozymes). Experimental error was low in RFLPs and RAPDs.
我们评估了叶绿体 DNA（cpDNA）、同工酶、单拷贝到低拷贝核 DNA（RFLP）和随机扩增多态性 DNA（RAPD）在 15 个各自的茄属块茎组和 S. palustre 以及 4 个 S. fernandezianum 访问的遗传距离的一致性。这些自交、二倍体（2n=24）且形态非常相似的类群构成了茄属块茎组的所有物种，这是一个不产块茎的物种组，与茄属块茎组（土豆及其野生亲缘）密切相关。遗传距离和多维标度结果表明，同工酶、RFLP 和 RAPD 之间在所有三个类群中具有一般一致性；cpDNA 显示 S. etuberosum 和 S. palustre 彼此之间比 S. fernandezianum 更相似。种间采样方差显示分辨率从同工酶（低）到 RAPD 到 RFLP（高）逐渐升高；而种内比较从 RFLP（低）到 RAPD（高；种内缺乏足够的同工酶变异性，无法进行所有同工酶的比较）。RFLP 和 RAPD 的实验误差较低。
