Limnologische Flußstation des Max-Planck-Instituts für Limnologie, Postfach 260, D-6407, Schlitz, FRG.
Microb Ecol. 1988 Jul;16(1):65-72. doi: 10.1007/BF02097405.
Samples were collected from organically polluted and unpolluted groundwater of sandy and gravelly deposits. After filtration onto polycarbonate filters (0.2μm pore size) the number of respiring bacteria was recorded by microscopically counting cells containing red INT-formazan spots, which characterize respiring bacteria. The total number of bacteria was simultaneously recorded by epifluorescence microscopy after staining with acridine orange. The number of respiring bacteria in the groundwater samples (55-490×10(3)/cm(3)) is within the range of values for other aquatic biotopes, but as the total number of bacteria in groundwater was usually higher, the proportion of respiring groundwater bacteria (0.66-7. 4%) was lower. Mainly larger bacteria, rods, and bacteria on particles could be identified as being active, whereas hardly any respiratory activity could be detected among small cocci and free interstitial bacteria. If the supply of dissolved organic matter (DOM) is adequate, the biomass of respiring bacteria correlates well with oxygen concentration, but there is no direct correlation between DOM concentration in groundwater and active bacterial biomass. Nor could any relationship be observed between the biomass of total and respiring bacteria, or between the quantity of respiring bacteria and heterotrophic bacterial activity.
从有机污染和未污染的沙质和砾质沉积物的地下水中采集了样本。在将其过滤到聚碳酸酯滤器（0.2μm 孔径）上之后，通过显微镜计数含有红色 INT-甲臜斑点的细胞来记录呼吸细菌的数量，这些斑点特征化了呼吸细菌。在用吖啶橙染色后，通过荧光显微镜同时记录细菌总数。地下水样本中（55-490×10(3)/cm(3)）的呼吸细菌数量处于其他水生生物栖息地的范围内，但由于地下水中的细菌总数通常较高，因此呼吸地下水细菌的比例（0.66-7.4%）较低。主要可以识别较大的细菌、棒状细菌和颗粒上的细菌为活性细菌，而几乎无法检测到小球菌和自由间隙细菌中的呼吸活性。如果溶解有机物（DOM）的供应充足，那么呼吸细菌的生物量与氧气浓度密切相关，但地下水中 DOM 浓度与活性细菌生物量之间没有直接关系。在总细菌和呼吸细菌的生物量之间，或在呼吸细菌的数量和异养细菌的活性之间，也观察不到任何关系。
