Staley J T
J Bacteriol. 1968 May;95(5):1921-42. doi: 10.1128/jb.95.5.1921-1942.1968.
Direct microscopic examination of natural freshwater samples reveals a variety of small microorganisms having elaborate cellular appendages. Several strains have been isolated from crude cultures containing low concentrations of organic nutrients. All of the isolates are procaryotic. They are aerobic chemoorganotrophs that require vitamins for growth. Because they cannot be assigned to any of the existing bacterial genera, two new genera are proposed: Ancalomicrobium for organisms which have several long appendages and which reproduce by budding; Prosthecomicrobium for organisms which have many short appendages tapering toward a blunt tip and which reproduce by binary fission. Gas vacuoles have been found in strains of each genus. The term prostheca is proposed for the rigid appendages of procaryotic cells bounded by the cell wall, and is defined to include the structures on these new bacteria, as well as the stalks of the caulobacters and the hyphae of the hyphomicrobia.
对天然淡水样本进行直接显微镜检查发现了各种具有复杂细胞附属物的小微生物。从含有低浓度有机养分的粗培养物中分离出了几个菌株。所有分离物都是原核生物。它们是需氧化学有机营养菌，生长需要维生素。由于它们不能归入任何现有的细菌属，因此提出了两个新属：Ancalomicrobium属用于具有几条长附属物并通过出芽繁殖的生物；Prosthecomicrobium属用于具有许多向钝尖逐渐变细的短附属物并通过二分裂繁殖的生物。在每个属的菌株中都发现了气泡。术语“菌柄”用于指由细胞壁界定的原核细胞的刚性附属物，并定义为包括这些新细菌上的结构，以及柄杆菌的柄和枝原体的菌丝。
