Department of Horticulture, University of Hawaii, 3190 Maile Way, 96822, Honolulu, HI, USA.
Plant Cell Rep. 1992 Aug;11(9):438-42. doi: 10.1007/BF00232686.
A method for the production of somatic embryos and subsequent plant regeneration for Anthurium andraeanum Linden ex André (Monocotyledonae) hybrids is described. Whole leaf blade explants, derived from plantlets grown in vitro, formed translucent embryogénic calli at their basal ends within one month of culture in the dark. Secondary somatic embryos formed frequently and without an intervening callus on surfaces of primary embryos. Embryogenesis was induced with three genotypes using a modified half-strength Murashige and Skoog (MS) medium supplemented with 1.0 to 4.0 mg l(-1) 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.33 to 1.0 mg l(-1) kinetin. A combination of 2% sucrose with 1% glucose in the medium favored embryogenesis over 3% sucrose alone. Whole leaf blades on medium solidified with 0.18% Gelrite produced more somatic embryos than leaves on medium with 0.7% Bacto-agar. Within two to three months after culture initiation, embryos were transferred to modified MS medium containing 0.2 mg l(-1) 6-benzyladenine (BA) and 2% sucrose and placed in the light for conversion into plantlets. Rooted plantlets were recovered and transferred into pots with tree fern fiber medium and grown in the greenhouse.
描述了一种生产安祖花（Anthurium andraeanum Linden ex André）杂种体细胞胚和随后植物再生的方法。从体外培养的小植株中获得的完整叶片外植体，在黑暗中培养一个月内，其基部形成了半透明的胚性愈伤组织。在初级胚胎表面上，次级体细胞胚频繁形成，而没有中间愈伤组织。使用改良的半强度 Murashige 和 Skoog (MS) 培养基，用 1.0 至 4.0 mg l(-1) 2,4-二氯苯氧乙酸 (2,4-D) 和 0.33 至 1.0 mg l(-1) 激动素对三种基因型进行诱导。培养基中 2%蔗糖与 1%葡萄糖的组合比单独使用 3%蔗糖更有利于胚胎发生。在 0.18% Gelrite 固化的培养基上的完整叶片比在含有 0.7%Bacto-琼脂的培养基上的叶片产生更多的体细胞胚。在培养起始后两到三个月内，将胚胎转移到含有 0.2 mg l(-1) 6-苄基腺嘌呤 (BA) 和 2%蔗糖的改良 MS 培养基中，并在光照下转化为小植株。生根的小植株被回收并转移到树蕨纤维培养基的盆中，并在温室中生长。
