Dansk Planteforædling A/S, Boelshøj, P.O. 19, DK-4660, St. Heddinge, Denmark.
Plant Cell Rep. 1993 Aug;12(10):537-40. doi: 10.1007/BF00233055.
Green plants were repeatedly regenerated from suspension-derived protoplasts of Kentucky blue grass (Poa pratensis L.) cv. "Geronimo". One suspension was capable of donating competent protoplasts during long-term culture i. e. 10-16 months after its establishment. The plating efficiency of the protoplasts from three different suspension lines varied from 0.004% in the lowest up to 1.5% in the highest responding line, using agarose-bedding in nutrition medium devoid of nurse or feeder cultures. Green plants germinated from polyembryos, which developed from 0.4-2.7% of the protoplast-derived microcolonies. A total of 127 plants were successfully transferred to soil.
绿色植物可由肯塔基蓝草（Poa pratensis L.） cv.“Geronimo”悬浮液原生质体反复再生。一个悬浮液在长期培养中（即在建立后 10-16 个月）有能力提供有活力的原生质体。使用不含护理或饲养培养物的营养培养基中的琼脂糖床，来自三个不同悬浮系的原生质体的平板效率从最低的 0.004%变化到最高的 1.5%响应线。从 0.4-2.7%的原生质体衍生的微集落中发育出的多胚珠萌发成绿色植物。总共成功地将 127 株植物转移到土壤中。
