Foundation for Agricultural Plant Breeding, P.O. Box 117, 6700 AC, Wageningen, The Netherlands.
Plant Cell Rep. 1989 Mar;7(8):644-7. doi: 10.1007/BF00272050.
Callus induction and plant regeneration were studied in 15 cultivars of the facultative apomictic species Poa pratensis L. (Kentucky bluegrass).The tissue culture responses of mature seeds and immature inflorescences were compared. Murashige and Skoog's (MS) medium, supplemented with 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) was used for callus induction and maintenance. Plants could be regenerated from compact and friable callus on MS medium devoid of 2,4-D. Plants were recovered from 14 cultivars at a high frequency (up to 79% of the callus cultures) when young inflorescences were used as the explant material and from only 3 cultivars, at a low frequency (up to 3%), with seeds. Somatic embryos were observed in callus cultures of many cultivars. Fully developed germinating somatic embryos were occasionally observed. Plant regeneration appeared to take place both via somatic embryogenesis and organogenesis. Plants were generally green but albino shoots developed at a low frequency from friable callus.
在 15 个可自由孤雌生殖的物种草地早熟禾（Poa pratensis L.）品种中，研究了愈伤组织诱导和植株再生。比较了成熟种子和未成熟花序的组织培养反应。使用 Murashige 和 Skoog（MS）培养基，添加 2 mg/L 2,4-二氯苯氧乙酸（2,4-D）进行愈伤组织诱导和维持。在不含 2,4-D 的 MS 培养基上，可从紧实和易碎的愈伤组织再生出植株。当使用幼花序作为外植体材料时，可从 14 个品种中以高频率（高达 79%的愈伤组织培养物）回收植株，而仅从 3 个品种中以低频率（高达 3%）回收植株。在许多品种的愈伤组织培养物中观察到体细胞胚。偶尔会观察到完全发育的萌发体细胞胚。植株再生似乎通过体细胞胚胎发生和器官发生发生。植株通常为绿色，但从易碎的愈伤组织中以低频率发育出白化苗。
