Lboratoire AEB, Université de Picardie, 33 rue Saint Leu, 80039, Amiens Cedex, France.
Plant Cell Rep. 1993 Sep;12(11):621-4. doi: 10.1007/BF00232811.
Agrobacterium-mediated transformation of sugarbeet (Beta vulgaris L.) was investigated for T-DNA transfer efficiency, using an intron containing β-glucuronidase gene. Preculture and coculture of hypocotyl and cotyledon explants with acetosyringone upon infection was studied. Seven seed lots which included several hundred genotypes, were screened, and were all susceptible to T-DNA transfer but with variable frequencies. Cotyledon explants were more readily transformed than those from hypocotyls. Transformation frequency of hypocotyl explants increased with acetosyringone. Both preculture treatment and acetosyringone improved transformation in cotyledon explants. Callus assayed with fluorometric procedures confirmed that the GUS gene had been transferred into sugarbeet.
农杆菌介导的甜菜（Beta vulgaris L.）转化研究了 T-DNA 转移效率，使用含有内含子的β-葡萄糖醛酸酶基因。研究了感染时用乙酰丁香酮对下胚轴和子叶外植体的预培养和共培养。筛选了包括数百个基因型的 7 个种子批，它们都容易接受 T-DNA 转移，但频率不同。子叶外植体比下胚轴外植体更容易转化。下胚轴外植体的转化频率随乙酰丁香酮的增加而增加。预培养处理和乙酰丁香酮都提高了子叶外植体的转化效率。用荧光测定法检测的愈伤组织证实 GUS 基因已被转移到甜菜中。
