Faculté des Sciences, Laboratoire A. E. B., Université de Picardie, 33 rue Saint leu, 80039, Amiens cedex, France.
Plant Cell Rep. 1992 Jul;11(7):329-33. doi: 10.1007/BF00233359.
A simple and reproducible protocol for regeneration of sugarbeet plants from hypocotyl expiants derived from 21 day-old-seedlings has been developed. Expiants were cultured on MS medium containing 0.3 mg/l N6-Benzylaminopurine, 0.1 mg/l Naphthalene Acetic Acid, 50 mg/l adenine and 0.5% (w/v) fructose, 0.5% (w/v) sucrose and 0.5% (w/v) glucose to induce the formation of organogenic calli (2.3% to 46.5% organogenic efficiency, depending on populations). Shoot formation was induced in callus cultures of more than 1600 genotypes. Physiological age affected culture response and different genotypes had different temperature optima for organogenesis. Following transfer of regenerated plants to the greenhouse, DNA determinations were made to study the stability of ploidy. Differences in ploidy were observed in plants derived from both shortterm and long-term callus cultures; diploid true-to-type regenerants were 96% and 83%, respectively, from shortterm and long-term callus cultures.
已开发出一种简单且可重复的甜菜植株再生方案，可从 21 日龄幼苗的下胚轴外植体中获得。外植体在含有 0.3 mg/L N6-苄基腺嘌呤、0.1 mg/L 萘乙酸、50 mg/L 腺嘌呤和 0.5%（w/v）果糖、0.5%（w/v）蔗糖和 0.5%（w/v）葡萄糖的 MS 培养基上培养，以诱导器官发生愈伤组织（取决于群体，器官发生效率为 2.3%至 46.5%）。在 1600 多个基因型的愈伤组织中诱导出芽的形成。生理年龄影响培养反应，不同基因型的器官发生最适温度不同。再生植株转移到温室后，进行 DNA 测定以研究倍性稳定性。在来自短期和长期愈伤组织培养的植物中观察到倍性差异；分别有 96%和 83%的二倍体真杂种再生体来自短期和长期愈伤组织培养。
