Biotechnology Department, Carlsberg Research Laboratory, Gl. Carlsbergvej, DK-2500, Copenhagen, Valby, Denmark.
Planta. 1990 Jan;180(2):272-7. doi: 10.1007/BF00194007.
Chymotrypsin inhibitor-2, a lysine-rich protein in the barley endosperm, has been localized at the ultrastructural level by immunocytochemistry in developing barley endosperm cells 14 days post anthesis. The protein is deposited in the protein bodies. Two morphologically distinct types of protein bodies, small spherical and large irregularly shaped, are present. Golgi-apparatus-derived vesicles whose content is labelled by chymotrypsin inhibitor-2 antibody-gold particles are observed at the Golgi complex and around the vacuoles. These observations indicate that the transport of the protein to the site of deposition is mediated by the Golgi apparatus.
胰凝乳蛋白酶抑制剂-2 是大麦胚乳中的一种富含赖氨酸的蛋白质，通过对授粉后 14 天的大麦胚乳细胞进行免疫细胞化学定位，在超微结构水平上进行了定位。该蛋白沉积在蛋白体中。存在两种形态上明显不同的蛋白体，小的球形和大的不规则形状。在高尔基复合体和液泡周围观察到高尔基器衍生的小泡，其内容物被胰凝乳蛋白酶抑制剂-2 抗体-金颗粒标记。这些观察结果表明，蛋白质向沉积部位的运输是由高尔基器介导的。
