Losvik Aleksandra, Beste Lisa, Mehrabi Sara, Jonsson Lisbeth
Department of Ecology, Environment and Plant Sciences, Stockholm University, 106 91 Stockholm, Sweden.
Int J Mol Sci. 2017 Jun 20;18(6):1317. doi: 10.3390/ijms18061317.
Aphids are phloem feeders that cause large damage globally as pest insects. They induce a variety of responses in the host plant, but not much is known about which responses are promoting or inhibiting aphid performance. Here, we investigated whether one of the responses induced in barley by the cereal aphid, bird cherry-oat aphid ( L.) affects aphid performance in the model plant L. A barley cDNA encoding the protease inhibitor was expressed in and aphid performance was studied using the generalist green peach aphid ( Sulzer). There were no consistent effects on aphid settling or preference or on parameters of life span and long-term fecundity. However, short-term tests with apterous adult aphids showed lower fecundity on three of the transgenic lines, as compared to on control plants. This effect was transient, observed on days 5 to 7, but not later. The results suggest that the protease inhibitor is taken up from the tissue during probing and weakly inhibits fecundity by an unknown mechanism. The study shows that a protease inhibitor induced in barley by an essentially monocot specialist aphid can inhibit a generalist aphid in transgenic Arabidopsis.
蚜虫是以韧皮部为食的害虫，在全球范围内造成巨大损害。它们会在寄主植物中引发多种反应，但对于哪些反应促进或抑制蚜虫生长知之甚少。在此，我们研究了谷类蚜虫——鸟樱桃燕麦蚜（Rhopalosiphum padi L.）在大麦中诱导产生的一种反应是否会影响模式植物拟南芥（Arabidopsis thaliana L.）中的蚜虫生长。在拟南芥中表达了一个编码蛋白酶抑制剂的大麦cDNA，并使用多食性的桃蚜（Myzus persicae Sulzer）研究蚜虫生长情况。对蚜虫的降落或偏好以及寿命和长期繁殖力参数均未产生一致影响。然而，对无翅成年蚜虫的短期测试表明，与对照植物相比，在三个转基因株系上蚜虫的繁殖力较低。这种影响是短暂的，在第5至7天观察到，但之后未再出现。结果表明，蛋白酶抑制剂在蚜虫取食时从组织中摄取，并通过未知机制微弱地抑制繁殖力。该研究表明，一种基本以单子叶植物为食的蚜虫在大麦中诱导产生的蛋白酶抑制剂能够在转基因拟南芥中抑制多食性蚜虫。
