Department of Applied Biological Science, College of Agriculture and Veterinary Medicine, Nihon University, Fujisaw, 252, Kanagawa, Japan.
Plant Cell Rep. 1993 Jan;12(2):66-9. doi: 10.1007/BF00241936.
5-Deoxy-(iso)flavonoids are biosynthesized from 6'-deoxychalcone (isoliquiritigenin). The coaction of a reductase with chalcone synthase (CHS) has been established in soybean cells to be responsible for the synthesis of 6'-deoxychalcone. Western blot analysis of crude extracts from cultured cells of Glycyrrhiza echinata, another member of the Leguminosae, revealed proteins which cross-react with an antiserum raised against the soybean reductase. DEAE-Cellulose chromatography of the extract yielded fractions which showed CHS activity but not deoxychalcone synthase activity, and these fractions were also negative in Western blot analysis. In contrast, fractions displaying positive signals with the antiserum were also able to synthesize 6'-deoxychalcone/5-deoxyflavanone. These results indicate that in G. echinata, too, synthesis of 6'-deoxychalcone is likely to be performed by the coaction of the reductase and CHS. Induction of the reductase and CHS by yeast extract treatment of the cells was demonstrated.
5-脱氧（异）黄酮是由 6'-去氧查尔酮（异甘草素）生物合成的。已经在大豆细胞中证实，一种还原酶与查尔酮合酶（CHS）的共同作用负责 6'-去氧查尔酮的合成。甘草细胞培养细胞粗提物的 Western blot 分析显示，与大豆还原酶抗血清发生交叉反应的蛋白质。提取物的 DEAE-纤维素层析得到显示 CHS 活性但没有去氧查尔酮合酶活性的部分，并且这些部分在 Western blot 分析中也是阴性的。相比之下，与抗血清显示阳性信号的部分也能够合成 6'-去氧查尔酮/5-脱氧黄烷酮。这些结果表明，在甘草中，6'-去氧查尔酮的合成也可能是由还原酶和 CHS 的共同作用来完成的。已经证明酵母提取物处理细胞会诱导还原酶和 CHS 的表达。
