Wingender R, Röhrig H, Höricke C, Wing D, Schell J
Max-Planck-Institut fur Züchtungsforschung, Köln, Federal Republic of Germany.
Mol Gen Genet. 1989 Aug;218(2):315-22. doi: 10.1007/BF00331284.
Four independent recombinant lambda clones hybridizing to parsley chalcone synthase (CHS) cDNA were isolated from a soybean (Glycine max) genomic library. Restriction fragment length polymorphism (RFLP) analysis indicated that the CHS gene family comprises six members. The CHS genes were found to be clustered with three genes on a 10 kb segment and pairs on others. DNA sequences of the 5'-, the coding-, and the 3' untranslated regions were determined for three different genes. A consensus alignment of the 5' regions revealed extensive homology between them for up to 150 bp upstream of the TATA box. Developmental regulation of CHS was observed in uninfected and in rhizobium-infected roots. Regulation at the level of transcription by different stimuli was investigated in the root, stem and cotyledons of soybean seedlings. Our results suggest a co-operative induction of CHS genes by wounding and elicitor treatment of cotyledons. The most rapid transcript accumulation, however, was observed in roots and stems. The induction of CHS genes by light was found to be UV dependent. A possible involvement of different members of the CHS gene family in response to elicitor versus UV treatment was analysed by the use of gene specific probes, and unexpectedly revealed that only CHS 1 transcription was induced by either elicitor or UV treatment of seedlings.
从大豆（Glycine max）基因组文库中分离出四个与欧芹查尔酮合酶（CHS）cDNA杂交的独立重组λ克隆。限制性片段长度多态性（RFLP）分析表明，CHS基因家族由六个成员组成。发现CHS基因在一个10 kb片段上与三个基因成簇，在其他片段上则成对存在。测定了三个不同基因的5'非翻译区、编码区和3'非翻译区的DNA序列。5'区域的一致性比对显示，在TATA框上游多达150 bp的区域之间存在广泛的同源性。在未感染和根瘤菌感染的根中观察到CHS的发育调控。在大豆幼苗的根、茎和子叶中研究了不同刺激在转录水平上的调控。我们的结果表明，子叶受伤和激发子处理可协同诱导CHS基因。然而，在根和茎中观察到转录本积累最为迅速。发现CHS基因受光诱导是依赖紫外线的。通过使用基因特异性探针分析了CHS基因家族不同成员在响应激发子与紫外线处理中的可能作用，意外发现只有CHS 1转录受到激发子或紫外线处理幼苗的诱导。
