Department of Horticulture, The Pennsylvania State University, 16802, University Park, PA, USA.
Plant Cell Rep. 1993 Jan;12(2):80-3. doi: 10.1007/BF00241939.
Callus cultures were established from Cephalotaxus harringtonia (Japanese plumyew) stem expiants cultured on Murashige and Skoog medium supplemented with 4.5 μM 2,4-dichlorophenoxyacetic acid and 0.05 βM 6-furfurylaminopurine. The inclusion of 4.9 μM 6-(γ,γ-dimethylallylamino) purine as the sole hormone significantly increased the growth rate of the callus. Organogenesis giving rise to both shoots and roots occurred upon transfer of the callus onto a hormonefree medium. Vitrification was common on all regenerated shoots cultured on Gelrite-containing medium. Regenerated roots were excised and established in McCown's woody plant medium. Doubling the phosphate and nitrate levels in the medium increased the growth of these root cultures.
愈伤组织培养物是从在补充有 4.5 μM 2,4-二氯苯氧乙酸和 0.05 βM 6-糠基氨基嘌呤的 Murashige 和 Skoog 培养基上培养的 Cephalotaxus harringtonia（日本扁柏）茎外植体建立的。将 4.9 μM 6-（γ，γ-二甲基烯丙基氨基）嘌呤作为唯一激素添加进去，可显著提高愈伤组织的生长速度。当将愈伤组织转移到无激素培养基上时，会发生导致芽和根形成的器官发生。在含有 Gelrite 的培养基上培养的所有再生芽上，玻璃化现象很常见。再生根被切除并在 McCown 的木本植物培养基中建立。将培养基中的磷酸盐和硝酸盐水平提高一倍可增加这些根培养物的生长。
