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World J Microbiol Biotechnol. 1995 Jul;11(4):461-7. doi: 10.1007/BF00364621.
Two key issues in the application of plant-cell-culture technology to the production of valuable secondary metabolites are reviewed: the selection of cell lines with suitable genetic, biochemical and physiological characteristics; and the optimization of bioreactor environments. Although great progress has been made in recent years in the design, selection and optimization of bioreactor hardware, optimization of environmental factors such as medium components, light irradiation and O2 supply needs detailed investigations for each case. With a better understanding of plant cell metabolism and physiology, further developments in cultivation processes, such as process integration and on-line monitoring and control, can be expected in the near future.
具有合适遗传、生化和生理特性的细胞系的选择；以及生物反应器环境的优化。尽管近年来在生物反应器硬件的设计、选择和优化方面取得了很大进展，但对于每种情况，仍需要详细研究诸如培养基成分、光照和 O2 供应等环境因素的优化。随着对植物细胞代谢和生理学的进一步了解，预计在不久的将来，培养过程的进一步发展，如过程集成和在线监测和控制，将会实现。
