Giménez-Gallego G, Fenoll C, Ramírez J M
Anal Biochem. 1986 Jan;152(1):29-34. doi: 10.1016/0003-2697(86)90113-2.
The light-harvesting B880 complex of Rhodospirillum rubrum was purified by a new method which allowed recovery of 66% of the amount present in the crude solubilized extract. Electrophoretic analysis of the isolated complex, followed by either Coomassie brilliant blue or silver staining, revealed only two low-molecular-weight polypeptides. When compared to a previously described preparation, the stability of the complex was considerably increased. In addition, the new procedure yielded B880 of higher purity as evidenced both by the decreased protein to pigment ratio (A280/A880 = 0.4) and by the absence of contaminants previously detected by silver staining or by an immunochemical method in other preparations. The most prominent of those contaminants were identified in this work as lipopolysaccharides of the bacterial outer membrane.
通过一种新方法纯化了红螺菌的捕光B880复合物，该方法可回收粗溶解提取物中66%的该复合物。对分离出的复合物进行电泳分析，随后用考马斯亮蓝或银染色，结果仅显示出两条低分子量多肽。与先前描述的制剂相比，该复合物的稳定性显著提高。此外，新方法产生的B880纯度更高，这一点可通过降低的蛋白质与色素比率（A280/A880 = 0.4）以及在其他制剂中通过银染色或免疫化学方法先前检测到的污染物不存在来证明。在这项工作中，这些污染物中最突出的被鉴定为细菌外膜的脂多糖。
