Instituto de Biología Celular CSIC, Velázquez 144, 28006, Madrid, Spain.
Photosynth Res. 1985 Jan;7(2):151-61. doi: 10.1007/BF00037005.
A solubilized preparation of the major Rhodospirillum rubrum antenna complex (B880) was obtained by a described procedure and its polypeptide composition was analyzed by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Only two polypeptides of molecular weights close to 7000 were detected after staining the gels with Coomassie brilliant blue. However, several other constituents could be visualized by silver staining or by an immunochemical method. When the preparation was chromatographed on Sephacryl, some of the resulting fractions exhibited the characteristic B880 absorption spectrum and contained only the two proteins that were detectable with Coomassie brilliant blue. In those fractions the A 280/A 880ratio was 0.4, which indicated a significant improvement of the bacteriochlorophyll to protein ratio over the unchromatographed preparation (A 280/A 880=0.7). Other chromatography fractions lacked bacteriochlorophyll and contained a carotenoid which seemed to be bound to protein. The macromolecular constituents present in these latter fractions differed from those associated to the purified B880 complex in their electrophoretic moblities and/or in their staining properties. That suggested the possible existence of a carotenoprotein that did not result from the B880 complex upon loss of bacteriochlorophyll.
已按照描述的程序获得了溶于水的主要红假单胞菌天线复合物（B880）的制剂，并通过聚丙烯酰胺凝胶电泳在十二烷基硫酸钠存在的情况下分析了其多肽组成。用考马斯亮蓝染色凝胶后，仅检测到两种接近 7000 分子量的多肽。然而，通过银染或免疫化学方法可以观察到其他几个成分。当制剂在 Sephacryl 上进行色谱分离时，一些得到的级分表现出特征性的 B880 吸收光谱，并且仅包含用考马斯亮蓝可检测到的两种蛋白质。在这些级分中，A280/A880 的比值为 0.4，这表明与未经过色谱分离的制剂相比（A280/A880=0.7），细菌叶绿素与蛋白质的比值有了显著提高。其他色谱级分缺乏细菌叶绿素，并且含有似乎与蛋白质结合的类胡萝卜素。在这些后一部分存在的大分子成分在电泳迁移率和/或染色特性方面与与纯化的 B880 复合物相关的成分不同。这表明可能存在一种不依赖于细菌叶绿素损失的与 B880 复合物相关的类胡萝卜蛋白。
