Department of Chemistry, University of New Mexico, 103 Clark Hall, 87131, Albuquerque, NM.
J Am Soc Mass Spectrom. 1996 Jan;7(1):114-7. doi: 10.1016/1044-0305(95)00646-X.
To determine the analytical utility of photodissociation as a general fragmentation technique for tandem mass spectrometry of organic ions, the ability to fragment those ions considered least likely to absorb photons efficiently was investigated. To this end, the ability to photodissociate ions of aliphatic compounds by using 193-nm photons has been studied. Three fragment ions, the C4H 9 (+) ion from n-hexane, the C4H 7 (+) ion from 2-hexene, and C4H 5 (+) from 2-hexyne, have been photodissociated. The fragmentation efficiencies for all three ions studied were between 25 and 45%. The photofragment ion spectrum for each precursor ion studied is made up of characteristic fragments. These spectra demonstrate the ability to photodissociate aliphatic ions that originate from both saturated and unsaturated molecules. This provides substantial hope that virtually all organic ions will be able to be photodissociated by using 193-nm photons.
为了确定光解作为串联质谱法中有机离子的一般碎裂技术的分析效用，研究了那些被认为最不可能有效吸收光子的离子的碎裂能力。为此，研究了使用 193nm 光子使脂肪族化合物的离子光解的能力。已经光解了正己烷的 C4H9(+)离子、2-己烯的 C4H7(+)离子和 2-己炔的 C4H5(+)离子这三个碎片离子。所研究的所有三种离子的碎裂效率都在 25%至 45%之间。每个所研究的前体离子的光碎片离子谱由特征碎片组成。这些谱图表明，能够使源自饱和和不饱和分子的脂肪族离子光解。这为几乎所有的有机离子都能够通过使用 193nm 光子进行光解提供了很大的希望。
