长寿命金属化尖端用于纳升电喷雾质谱。
Long-lived metallized tips for nanoliter electrospray mass spectrometry.
机构信息
Chemistry Department, Cornell University, Ithaca, New York, USA.
出版信息
J Am Soc Mass Spectrom. 1996 Dec;7(12):1270-2. doi: 10.1016/S1044-0305(96)00152-3.
Sheathless electrospray at nL/min flow rates combined with Fourier-transform mass spectrometry has made possible high resolving power (>50,000) mass spectra of subattomole samples of >8 kDa proteins separated by capillary electrophoresis (Valaskovic, G. A.; Kelleher, N. L.; McLafferty, F. W. Science, 1996, 273, 1199-1202). However, for this new method the mechanical stability of the thin (35 to 100 nm) gold film electrodes has limited tip lifetime to 15 to 30 min. A technique for SiO x coating of the gold is described that provides a steady ion current (±10 pA) for 1 to 2 h, even with arcs or interruptions of the electrospray voltage.
无鞘层电喷雾在纳升/分钟的流速下结合傅里叶变换质谱，已经可以实现纳升级 (<8 kDa) 蛋白质样品的高分辨率 (>50,000) 质谱，这些蛋白质是通过毛细管电泳分离的（Valaskovic，G. A.；Kelleher，N. L.；McLafferty，F. W.，《科学》，1996，273，1199-1202）。然而，对于这种新方法，薄（35 至 100nm）金膜电极的机械稳定性将尖端寿命限制在 15 至 30 分钟。本文描述了一种 SiO x 涂覆金的技术，即使在电喷雾电压中断或电弧的情况下，也能提供稳定的离子电流（±10pA）持续 1 至 2 小时。
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