Protein and Peptide Group, European Molecular Biology Laboratory (EMBL), Meyerhof-strasse 1, D-69012, Heidelberg, Germany.
J Am Soc Mass Spectrom. 1996 Feb;7(2):150-6. doi: 10.1016/1044-0305(95)00626-5.
A nano electrospray (NanoES)/ion trap combination was developed to take advantage of the long spraying time of the NanoES source (Wilm, M. S., Mann, M. Int. J. Mass Spectrom. Ion Processes 1994, 136, 167-180) for extensive experiments on the ion trap. The low flow rate associated with the NanoES allows for optimization of experimental conditions and data acquisition for more than 30 min with 1 µL of solution. Thus, even time-consuming experiments, such as multiple fragmentation steps or the sequencing of many components in a mixture, can be performed with very small volumes of sample. Stored waveform inverse Fourier transformation (SWIFT) signals were used during the injection period to accumulate ions of low intensity and to improve the dynamic range of the quadrupole ion trap. To sequence peptides, a combination of nozzle-skimmer fragmentation, SWIFT accumulation of a single fragment to high intensity, and a second fragmentation step was employed to obtain complete sequence information. Unseparated peptide mixtures were infused and weak portions of the spectrum were enhanced by using the SWIFT method, followed by fragmentation of various accumulated peptide ions.
开发了一种纳喷雾（NanoES）/离子阱组合，以利用 NanoES 源的长喷雾时间（Wilm，M. S.，Mann，M. Int. J. Mass Spectrom. Ion Processes 1994，136，167-180），以便在离子阱上进行广泛的实验。与 NanoES 相关的低流速允许优化实验条件和数据采集，使用 1 µL 溶液可进行超过 30 分钟的实验。因此，即使是耗时的实验，如多次碎片化步骤或混合物中许多成分的测序，也可以使用非常小的样品体积进行。在注入期间使用存储波形逆傅里叶变换（SWIFT）信号来积累低强度的离子，并提高四极离子阱的动态范围。为了对肽进行测序，采用了喷嘴-分流器碎片化、SWIFT 对单个片段进行高强度累积以及第二步碎片化的组合，以获得完整的序列信息。通过使用 SWIFT 方法对未分离的肽混合物进行注入，并增强谱的弱部分，然后对各种累积的肽离子进行碎片化。
