Moqbel R, Walsh G M, Macdonald A J, Kay B
Clin Allergy. 1986 Jan;16(1):73-83. doi: 10.1111/j.1365-2222.1986.tb01956.x.
Immunological release of histamine and lipid mediators is known to occur when basophils, contained in whole blood human leucocytes, are incubated with anti-IgE (reversed anaphylaxis). In the present study we show that IgE-dependent stimulation of basophils was associated with activation of bystander eosinophils and neutrophils, as assessed by enhanced complement (C3b) and IgG (Fc) rosettes, and increased cytotoxicity for complement-coated schistosomula of Schistosoma mansoni. These changes in eosinophil and neutrophil function were totally inhibited in a dose-dependent fashion by prior incubation with disodium cromoglycate (DSCG). In all in vitro systems examined, complete inhibition of enhancement was observed with concentrations as low as 10(-7) moles/l. In contrast, DSCG had no effect on histamine release, or the percentage of rosettes or cytotoxicity prior to anti-IgE stimulation. These results suggest that DSCG inhibits activation of inflammatory cells consequent to an IgE-dependent stimulus.
已知当全血人类白细胞中的嗜碱性粒细胞与抗IgE一起孵育时（反向过敏反应），会发生组胺和脂质介质的免疫释放。在本研究中，我们发现，通过增强补体（C3b）和IgG（Fc）花环检测，以及对曼氏血吸虫补体包被的尾蚴细胞毒性增加评估，嗜碱性粒细胞的IgE依赖性刺激与旁观者嗜酸性粒细胞和中性粒细胞的激活有关。嗜酸性粒细胞和中性粒细胞功能的这些变化，通过预先与色甘酸钠（DSCG）孵育，以剂量依赖方式被完全抑制。在所有检测的体外系统中，浓度低至10（-7）摩尔/升时，增强作用即被完全抑制。相比之下，DSCG对抗IgE刺激前的组胺释放、花环百分比或细胞毒性没有影响。这些结果表明，DSCG抑制IgE依赖性刺激导致的炎症细胞激活。
