通过限制性内切核酸酶处理以及地霉素A/4',6-二脒基-2-苯基吲哚（DAPI）染色对印度麂（赤麂）染色体进行异染色质表达分析

Expression of heterochromatin by restriction endonuclease treatment and distamycin A/DAPI staining of Indian muntjac (Muntiacus muntjak) chromosomes.

作者信息

Babu A, Verma R S

出版信息

Cytogenet Cell Genet. 1986;41(2):96-100. doi: 10.1159/000132210.

PMID:2420537

Abstract

The constitutive heterochromatin of the Indian muntjac (Muntiacus muntjak) was examined following digestion with various restriction endonucleases (AluI, HaeIII, HinfI, and MboI), as well as by selective fluorescence staining with distamycin A plus 4'-6-diamidino-2-phenylindole. Distinct areas within the C-bands were found to have characteristic staining patterns which were more conspicuous in the sex chromosomes. Two dot-like structures resistant to AluI were found in the X and Y1 chromosomes in the same position as the nucleolus organizer regions.

摘要

在用各种限制性内切酶（AluI、HaeIII、HinfI和MboI）消化后，以及用偏端霉素A加4′-6-二脒基-2-苯基吲哚进行选择性荧光染色后，对印度麂（赤麂）的组成型异染色质进行了检查。发现C带内的不同区域具有特征性的染色模式，在性染色体中更为明显。在X和Y1染色体上与核仁组织区相同的位置发现了两个对AluI有抗性的点状结构。