Babu A, Verma R S
Histochem J. 1986 Jun;18(6):329-33. doi: 10.1007/BF01675211.
The heterogeneity of the C-band of human chromosome 1 has been evaluated using several selective staining methods: C-banding (CBG), distamycin A plus 4-6-diamidino-2-phenylindole (DA/DAPI) and Giemsa G-11 pattern following the treatment with the restriction endonucleases AluI and HaeIII. The bands produced by each method are characteristic but not identical. The total C-band is resistant to AluI treatment. The bands induced by HaeIII and the one stained by DA/DAPI are markedly similar but smaller than the C-band. The G-11 technique stains yet smaller regions than those of HaeIII and DA/DAPI. Depending on the expression of staining properties, the C-band of chromosome 1 usually consists of three subdivisions: the proximal, intermediate and distal regions, suggesting an extremely heterogeneous nature. The staining variations between different regions are further substantiated by studies of a reciprocal translocation where the proximal region and the remaining C-band of chromosome 1 are separate.
已使用几种选择性染色方法评估了人类1号染色体C带的异质性：C显带（CBG）、放线菌素A加4,6-二脒基-2-苯基吲哚（DA/DAPI）以及在用限制性核酸内切酶AluI和HaeIII处理后进行吉姆萨G-11染色模式分析。每种方法产生的带纹具有特征性但并不相同。总C带对AluI处理具有抗性。HaeIII诱导产生的带纹与DA/DAPI染色的带纹明显相似，但比C带小。G-11技术染色的区域比HaeIII和DA/DAPI染色的区域更小。根据染色特性的表现，1号染色体的C带通常由三个细分区域组成：近端、中间和远端区域，这表明其具有极其异质的性质。对一个相互易位的研究进一步证实了不同区域之间的染色差异，在该易位中，1号染色体的近端区域和其余C带是分开的。
