Levels of uracil DNA glycosylase and AP endonuclease in murine B- and T-lymphocytes do not change with age.

Two DNA repair enzyme activities, uracil DNA glycosylase and AP endonuclease, were measured in extracts of T- and B-lymphocytes isolated from mice ranging in age from 3 to 24 months. T- and B-lymphocytes had roughly equal levels of AP endonuclease which did not change appreciably with age. T-lymphocytes had roughly twice as high a level of uracil DNA glycosylase as B-lymphocytes; these levels were not affected by age either. This constancy with age contrasts dramatically with increases in both enzymes--roughly 3-fold on a protein basis or 50-fold on a per cell basis--in a transformed line (MPC-11) derived from a carcinogen-induced lymphocytoma. These results are similar to those obtained with cultured murine fibroblasts, wherein a relative constancy was noted with passage of non-transformed cells, followed by dramatic changes upon transformation (La Belle, M & Linn, S, Mutat res 132 (1984) 51). Hence these enzyme assays do not support the notion of a drop in base excision DNA repair capacity as being a causative factor in aging, but suggest instead that DNA repair properties might differ dramatically in transformed vs non-transformed cells.