胡萝卜细胞中的碱基切除修复。尿嘧啶-DNA糖基化酶和脱嘌呤/脱嘧啶内切脱氧核糖核酸酶的部分纯化及特性分析

Base-excision repair in carrot cells. Partial purification and characterization of uracil-DNA glycosylase and apurinic/apyrimidinic endodeoxyribonuclease.

作者信息

Talpaert-Borlè M, Liuzzi M

出版信息

Eur J Biochem. 1982 Jun;124(3):435-40.

Abstract

Uracil-DNA glycosylase and apurinic/apyrimidinic (AP) endodeoxyribonuclease have been purified from cultured carrot cells. The two enzymes, separated by affinity chromatography on Sepharose-poly(rU), were found to have properties similar to those of the homologous bacterial and mammalian enzymes. The action of AP endodeoxyribonuclease on (dA)230 . (dT, dU)230 partially depyrimidinated by uracil-DNA glycosylase suggests that these two enzymes might act successively to initiate the repair of uracil-containing DNA.

摘要

尿嘧啶-DNA糖基化酶和脱嘌呤/脱嘧啶（AP）内切脱氧核糖核酸酶已从培养的胡萝卜细胞中纯化出来。通过在琼脂糖-聚（rU）上进行亲和层析分离得到的这两种酶，其性质与同源的细菌和哺乳动物酶相似。AP内切脱氧核糖核酸酶对经尿嘧啶-DNA糖基化酶部分脱嘧啶的（dA）230·（dT，dU）230的作用表明，这两种酶可能相继作用以启动含尿嘧啶DNA的修复。