Mohammadzadeh Ghorban, Nikzamir Abdolrahim, Mohammadi Javad, Pourdashti Sara, Shabazian Hajeh, Latifi Seyed-Mahmoud
Hyperlipidemia Research Center, Department of Clinical Biochemistry, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Arch Iran Med. 2013 Nov;16(11):636-41.
Leptin, an adipocyte-derived hormone, has a pivotal role in the regulation of body weight through acting on its specific leptin receptor (LEPR). The 223A/G polymorphism of the LEPR gene is one of the most common polymorphism in all populations. In this study, we aimed to investigate the impact of the 223A/G polymorphism of the LEPR gene on serum levels of leptin in type 2 diabetes mellitus (T2DM) in a sample of Iranian population.
One hundred and forty-four T2DM patients were screened and compared to 147 healthy controls. The 223A/G LEPR polymorphism was genotyped using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP). The serum levels of leptin were measured.
The mean serum levels of leptin in T2DM patients were significantly higher than that of healthy control subjects; 22.90 ng/ml (95 % confidence interval [CI] = 20.79 - 25.23) vs. 8.70 ng/ml (95 % CI = 7.87 - 9.63). The genotypes (AA, AG, and GG) distributions of the 223A/G polymorphism were 55.5 %, 41 %, and 3.5 % in T2DM and 54.4 %, 42.2 %, and 3.4 % in healthy controls. The results showed no significant differences in the 223A/G LEPR genotype and allele frequencies between T2DM and control subjects (χ2 = 0.043, P = 0.979 and χ2 = 0.003, P = 0.957), respectively. In addition, the serum leptin levels were markedly higher in subjects with GG genotype than those with AG or GG genotype only in T2DM CONCLUSION: The 223A/G LEPR gene polymorphism is associated with markedly increased serum leptin levels in T2DM. However, no differences were determined in genotype and allele frequencies between T2DM patients and control subjects.
瘦素是一种由脂肪细胞分泌的激素，通过作用于其特定的瘦素受体（LEPR）在体重调节中起关键作用。LEPR基因的223A/G多态性是所有人群中最常见的多态性之一。在本研究中，我们旨在调查伊朗人群样本中LEPR基因223A/G多态性对2型糖尿病（T2DM）患者血清瘦素水平的影响。
对144例T2DM患者进行筛查，并与147例健康对照进行比较。采用聚合酶链反应（PCR）结合限制性片段长度多态性（RFLP）对223A/G LEPR多态性进行基因分型。检测血清瘦素水平。
T2DM患者血清瘦素平均水平显著高于健康对照者；分别为22.90 ng/ml（95%置信区间[CI]=20.79 - 25.23）和8.70 ng/ml（95%CI = 7.87 - 9.63）。223A/G多态性的基因型（AA、AG和GG）分布在T2DM患者中分别为55.5%、41%和3.5%，在健康对照中分别为54.4%、42.2%和3.4%。结果显示，T2DM患者与对照者之间223A/G LEPR基因型和等位基因频率无显著差异（χ2 = 0.043，P = 0.979和χ2 = 0.003，P = 0.957）。此外，仅在T2DM患者中，GG基因型受试者的血清瘦素水平明显高于AG或AA基因型受试者。结论：223A/G LEPR基因多态性与T2DM患者血清瘦素水平显著升高有关。然而，T2DM患者与对照者之间的基因型和等位基因频率无差异。
