Dere W H, Rapoport B
Mol Cell Endocrinol. 1986 Mar;44(3):195-9. doi: 10.1016/0303-7207(86)90124-3.
The purpose of this study was to determine the role of cAMP in the growth of FRTL and FRTL5 cells, 2 continuous cultured thyroid lines. TSH, at concentrations similar to those reported to induce growth in primary dog thyroid cultures, played an essential role for growth. Stimulators of adenylate cyclase, cholera toxin and forskolin, and cAMP analogues, dibutyryl cAMP and 8-bromo cAMP, mimicked the effect of TSH in both groups of cultured cells. The present data confirm the role of TSH in controlling growth of both cell lines and suggest that cAMP is an essential intracellular mediator of TSH action.
本研究的目的是确定环磷酸腺苷（cAMP）在两种连续培养的甲状腺细胞系FRTL和FRTL5细胞生长中的作用。促甲状腺激素（TSH）在与报道的能诱导原代犬甲状腺培养物生长的浓度相似时，对生长起着至关重要的作用。腺苷酸环化酶的刺激剂霍乱毒素和福斯高林，以及cAMP类似物二丁酰cAMP和8-溴cAMP，在两组培养细胞中都模拟了TSH的作用。目前的数据证实了TSH在控制这两种细胞系生长中的作用，并表明cAMP是TSH作用的一种重要细胞内介质。
