Wynford-Thomas D, Smith P, Williams E D
Mol Cell Endocrinol. 1987 May;51(1-2):163-6. doi: 10.1016/0303-7207(87)90130-4.
The role of cyclic AMP (cAMP) as an intracellular growth signal in thyroid epithelial (follicular) cells has been studied using primary suspension cultures of rat thyroid follicles closely resembling the in vivo state. TSH at 0.1 mU/ml in the presence of insulin (0.08 microgram/ml) induced a 9.6-fold increase in [3H]thymidine incorporation with a peak after 48-72 h. The response to the cAMP elevating agent cholera toxin (10 ng/ml) was identical both in timing, magnitude and dependence on insulin. A lower amplitude response occurred with forskolin. The data further support the conclusion that elevation of intracellular cAMP concentration is a major, if not the only, signal required for the proliferative response of the follicular cell to its physiological mitogen, TSH.
利用与体内状态极为相似的大鼠甲状腺滤泡原代悬浮培养物，研究了环磷酸腺苷（cAMP）作为甲状腺上皮（滤泡）细胞内生长信号的作用。在存在胰岛素（0.08微克/毫升）的情况下，0.1毫单位/毫升的促甲状腺激素（TSH）使[3H]胸苷掺入量增加了9.6倍，在48 - 72小时后达到峰值。对cAMP升高剂霍乱毒素（10纳克/毫升）的反应在时间、幅度和对胰岛素的依赖性方面均相同。福斯高林引起的反应幅度较低。这些数据进一步支持了这样的结论，即细胞内cAMP浓度的升高是滤泡细胞对其生理性有丝分裂原TSH增殖反应所需的主要信号，即便不是唯一信号。
