Triebel F, de Roquefeuil S, Blanc C, Charron D J, Debre P
Hum Immunol. 1986 Mar;15(3):302-15. doi: 10.1016/0198-8859(86)90005-4.
The expression of interleukin 2 (IL2) receptor and HLA-DR, DQ, and DP antigens on the surface of four diphtheria toxoïd (DT)-specific T lymphocyte clones (TLC) and two TLC specific for an allogeneic EBV-transformed cell line was investigated with the use of monoclonal antibodies (MoAbs) that recognize defined molecules or epitopes. Incubation of a resting TLC with IL2 resulted in a 10- to 30-fold increase in the level of DR, DQ, and Tac antigen expression. On the other hand, incubation of an activated TLC with IL2 decreased for 1 to 6 hr the level of expression of these three antigens. Anti-FA MoAbs did not react with any of the TLC tested suggesting that the expression of DR, DQ, and DP antigens is dissociated on activated TLC. Surface-marker analysis with anti-DR MoAbs indicated that DR epitopes were differently expressed at some activation stages of the TLC. Functional studies showed that activated TLC can stimulate in MLR, AMLR, and PLT. These proliferative responses were inhibited by preincubating the TLC with anti-DR MoAbs suggesting that the stimulatory determinants were predominantly DR molecules. In addition, some TLC can act as antigen presenting cells in DT-specific proliferative responses. These results indicate that MHC class II molecules on activated T lymphocytes may be relevant for the control of specific immunologic responses in vivo.
利用识别特定分子或表位的单克隆抗体（MoAbs），研究了四种白喉类毒素（DT）特异性T淋巴细胞克隆（TLC）以及两种针对同种异体EBV转化细胞系的TLC表面白细胞介素2（IL2）受体和HLA - DR、DQ及DP抗原的表达情况。静息TLC与IL2孵育导致DR、DQ和Tac抗原表达水平增加10至30倍。另一方面，活化的TLC与IL2孵育1至6小时会使这三种抗原的表达水平降低。抗FA MoAbs与所测试的任何TLC均无反应，这表明在活化的TLC上DR、DQ和DP抗原的表达是分离的。用抗DR MoAbs进行的表面标志物分析表明，DR表位在TLC的某些活化阶段有不同表达。功能研究表明，活化的TLC可在混合淋巴细胞反应（MLR）、自体混合淋巴细胞反应（AMLR）和血小板裂解物反应（PLT）中发挥刺激作用。预先用抗DR MoAbs孵育TLC可抑制这些增殖反应，这表明刺激决定簇主要是DR分子。此外，一些TLC在DT特异性增殖反应中可作为抗原呈递细胞。这些结果表明，活化T淋巴细胞上的MHC II类分子可能与体内特异性免疫反应的控制有关。
