Triebel F, De Roquefeuil S, Bernard A, Blanc C, Debre P
Hum Immunol. 1986 Jul;16(3):221-33. doi: 10.1016/0198-8859(86)90050-9.
The modulation of antigen expression on the surface of TLC by different differentiation inducers as well as the inhibition of the TLC proliferative response by specific MoAbs may lead to a clarification of the role of various surface molecules studied in antigen-specific T-cell response. We investigated the expression of CD 1-8 antigens on the surface of diphtheria toxoid or varidase specific TLC with a series of MoAb. CD1 and CD8 antigens were not expressed on the proliferative TLC. CD2, CD3, CD4, and CD5 antigens were homogeneously expressed on all TLC in contrast to CD6 and CD7 antigens which were present on only a fraction of the cells in a given TLC. In functional assays, anti-CD2, anti-DR VI-15C, and anti-CD25 MoAbs blocked the proliferative response to DT and anti-CD3, anti-CD4, anti-D44 MoAbs had intermediate inhibition effects. Anti-CD5, anti-CD6, and anti-CD7 MoAbs did not inhibit the proliferative response. Surface marker analysis revealed that the expression of CD2 to CD7 antigens (and also CD25) may be modified following incubation of the TLC with TPA or sodium butyrate but not with 5-azacytidine. TPA greatly decreased the expression of CD3 and CD4 antigens after a 6-hr exposure. Preincubation of TLC cells with TPA inhibited the mitogenic effect of anti-CD3 MoAbs on TLC cells in proliferative assays, but did not inhibit their capacity to proliferate in response to DT despite low levels of CD3 and CD4.
不同分化诱导剂对TLC表面抗原表达的调节以及特异性单克隆抗体对TLC增殖反应的抑制，可能有助于阐明在抗原特异性T细胞反应中所研究的各种表面分子的作用。我们用一系列单克隆抗体研究了白喉类毒素或瓦里idase特异性TLC表面CD1 - 8抗原的表达。增殖性TLC上未表达CD1和CD8抗原。与CD6和CD7抗原仅存在于给定TLC中一部分细胞上不同，CD2、CD3、CD4和CD5抗原在所有TLC上均均匀表达。在功能试验中，抗CD2、抗DR VI - 15C和抗CD25单克隆抗体阻断了对DT的增殖反应，抗CD3、抗CD4、抗D44单克隆抗体具有中等抑制作用。抗CD5、抗CD6和抗CD7单克隆抗体未抑制增殖反应。表面标志物分析显示，TLC与佛波酯（TPA）或丁酸钠孵育后，CD2至CD7抗原（以及CD25）的表达可能会发生改变，但与5 - 氮杂胞苷孵育则不会。暴露6小时后，TPA大大降低了CD3和CD4抗原的表达。在增殖试验中，用TPA预孵育TLC细胞可抑制抗CD3单克隆抗体对TLC细胞的促有丝分裂作用，但尽管CD3和CD4水平较低，却不抑制它们对DT的增殖能力。
