Miroli A A, James B M, Spitz M
J Immunol Methods. 1986 Apr 3;88(1):91-6. doi: 10.1016/0022-1759(86)90055-4.
A simple, rapid and economical method for the enrichment of basophils from peripheral blood with high recovery is described. Peripheral blood was layered over a Ficoll-Paque cushion and centrifuged. The Ficoll-Paque layer was aspirated taking care not to disturb the interphase and the buffy coat. The Ficoll-Paque layer contained most of the basophils. A recovery of 82% (65-95%) and a 23.5-fold enrichment of basophils with their morphology and biological activity preserved was achieved.
本文描述了一种简单、快速且经济的从外周血中富集嗜碱性粒细胞的方法，回收率高。将外周血铺在Ficoll-Paque垫层上并离心。吸取Ficoll-Paque层，注意不要扰动界面和血沉棕黄层。Ficoll-Paque层含有大部分嗜碱性粒细胞。嗜碱性粒细胞的回收率为82%（65%-95%），且形态和生物活性得以保留，富集了23.5倍。
