Yamazaki H, Takahashi T, Sano T
Thromb Diath Haemorrh. 1975 Sep 30;34(1):94-105.
Platelet aggregability was measured using platelet rich plasma (PRP) collected from 197 clinical cases including 52 healthy volunteers. In 31 patients of acute stage of thrombosis (within 2 weeks from the onset), a significant enhancement of platelet aggregation measured 5 min after an addition of 3 and 10 muM ADP or 0.1 and 1 mug/ml of adrenaline to PRP (p less than 0.05, compared to the healthy group). Also a significant enhancement of secondary aggregation induced by adrenaline was observed (p less than 0.05). The enhancement was especially marked in the response induced by adrenaline. Such an enhancement was not observed in patients in the recovery stage of thrombosis, hypertension, angina pectoris and other miscellaneous diseases. There was no difference in the parameters related to the velocity of aggregation or intensity of primary aggregation between the diseased and the healthy group. In response induced by collagen (bovine achilles tendon, 0.3 and 1 mg/ml) any difference in the aggregation curve was not observed between the diseased and the healthy group. Such findings suggest a presence of an enhancement of ADP-release mechanism of platelets in acute thrombosis. Aslo a significance of adrenaline-induced platelet aggregation was proposed to detect platelet functions for analysis of mechanism of thromboembolic disorders.
使用从包括52名健康志愿者在内的197例临床病例中采集的富血小板血浆（PRP）来测量血小板聚集性。在31例血栓形成急性期患者（发病后2周内）中，向PRP中添加3和10μM ADP或0.1和1μg/ml肾上腺素后5分钟测量的血小板聚集显著增强（与健康组相比，p<0.05）。还观察到肾上腺素诱导的二次聚集显著增强（p<0.05）。这种增强在肾上腺素诱导的反应中尤为明显。在血栓形成恢复期、高血压、心绞痛和其他杂病患者中未观察到这种增强。患病组和健康组之间在与聚集速度或初级聚集强度相关的参数上没有差异。在由胶原蛋白（牛跟腱，0.3和1mg/ml）诱导的反应中，患病组和健康组之间在聚集曲线上未观察到任何差异。这些发现表明急性血栓形成中血小板的ADP释放机制增强。还提出了肾上腺素诱导的血小板聚集对于检测血小板功能以分析血栓栓塞性疾病机制的意义。
