在选择具有双胎排卵和分娩的牛中，IGF2 受体在调节 2 个优势卵泡选择中的可能作用。
Possible role of IGF2 receptors in regulating selection of 2 dominant follicles in cattle selected for twin ovulations and births.
机构信息
Department of Animal Science, Oklahoma State University, 114 Animal Science Bldg., Stillwater, OK 74078, USA.
出版信息
Domest Anim Endocrinol. 2013 Nov;45(4):187-95. doi: 10.1016/j.domaniend.2013.09.001. Epub 2013 Oct 4.
Abundance of IGF-2 receptor (IGF2R), FSH receptor (FSHR), and LH receptor (LHCGR) mRNA in granulosa cells (GCs) or theca cells (TCs) or both cells as well as estradiol (E2), progesterone (P4), and androstenedione concentrations in follicular fluid were compared in cows genetically selected (Twinner) or not selected (control) for multiple ovulations and twin births. Cows were slaughtered at day 3 to 4 (day 3) and day 5 to 6 (day 5) of an estrous cycle, and ovaries, follicular fluid, GCs, and TCs were collected. The two largest (F1 and F2) E2-active (EA) and E2-inactive (EI) follicles were selected according to their E2-to-P4 ratio and diameter. Androstenedione levels in EA F1 and F2 follicles were 5-fold greater (P < 0.05) in Twinner cows than in control cows on day 3 but did not differ on day 5. Twinner cows also had greater (P < 0.05) E2 and P4 concentrations, whereas steroid levels in EI follicles did not differ (P > 0.10) between genotypes. In EA F2 follicles, IGF2R levels in GCs were greater (P < 0.05) in control cows than in Twinner cows on day 3 and day 5, whereas IGF2R mRNA in TCs did not differ (P > 0.10). On day 3, FSHR mRNA levels were greater (P < 0.05) in GCs of EA F1 and EI F2 follicles of control cows than of Twinner cows. LH receptor mRNA expression was less in GCs and greater in TCs of EA F2 follicles in control cows than in Twinner cows (P < 0.05). We hypothesize that reduced GC IGF2R expression in F2 follicles of Twinner cows may play a role in the development of 2 or more dominant follicles.
比较了为多排卵和双胞胎出生而进行基因选择（双生子）或未选择（对照）的奶牛的颗粒细胞（GC）或膜细胞（TC）或两种细胞中的胰岛素样生长因子 2 受体（IGF2R）、促卵泡激素受体（FSHR）和促黄体激素受体（LHCGR）mRNA 的丰度，以及卵泡液中的雌二醇（E2）、孕酮（P4）和雄烯二酮浓度。奶牛在发情周期的第 3 天至第 4 天（第 3 天）和第 5 天至第 6 天（第 5 天）被屠宰，采集卵巢、卵泡液、GC 和 TC。根据其 E2 与 P4 比值和直径选择两个最大的（F1 和 F2）E2 活性（EA）和 E2 非活性（EI）卵泡。第 3 天，双生子奶牛的 EA F1 和 F2 卵泡中的雄烯二酮水平比对照奶牛高 5 倍（P<0.05），但第 5 天没有差异。双生子奶牛的 E2 和 P4 浓度也较高（P<0.05），而 EI 卵泡中的类固醇水平在基因型之间没有差异（P>0.10）。在 EA F2 卵泡中，第 3 天和第 5 天，GC 中的 IGF2R 水平在对照奶牛中高于双生子奶牛（P<0.05），而 TC 中的 IGF2R mRNA 没有差异（P>0.10）。第 3 天，在对照奶牛的 EA F1 和 EI F2 卵泡的 GC 中，FSHR mRNA 水平高于双生子奶牛（P<0.05）。在对照奶牛的 EA F2 卵泡的 GC 和 TC 中，LH 受体 mRNA 表达较低，而在双生子奶牛中则较高（P<0.05）。我们假设，双生子奶牛的 F2 卵泡中 GC IGF2R 表达减少可能在 2 个或更多优势卵泡的发育中起作用。
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