Tim-3启动子区域多态性与汉族女性不明原因复发性自然流产的关系。
The relationship between polymorphisms in the promoter region of Tim-3 and unexplained recurrent spontaneous abortion in Han Chinese women.
作者信息
Shen Yang, Wang Chen, Hong Dun, Zeng Baojin, Fang Congcheng, Yuan Chiting, Fan Lilong, Lv Haiyan, Zhu Min
机构信息
The Public Laboratory, Taizhou Hospital of Zhejiang Province, Wenzhou Medical College, Linhai, Zhejiang 317000, China.
出版信息
Reprod Biol Endocrinol. 2013 Nov 11;11:104. doi: 10.1186/1477-7827-11-104.
BACKGROUND
Recurrent spontaneous abortion (RSA) refers to 2 or more consecutive pregnancy losses, and RSA with unknown causes is called unexplained recurrent spontaneous abortion (URSA). Tim-3, a subtype of the T-cell immunoglobulin domain and mucin domain (Tim) protein family, might be an important regulatory molecule that plays a pivotal role in URSA, which might be triggered mostly by Th1/Th2 immune deviation. To understand the etiology and pathogenesis of URSA in Han Chinese women, we investigated the association between polymorphisms of rs10053538 and rs10515746 in the promoter of Tim-3 and the risk of URSA in Han Chinese women.
METHODS
One hundred and forty-eight women with RSA resulting in still birth were enrolled in the URSA group. We performed tests to rule out congenital reproductive system malformation, reproductive system tumor, endocrine dyscrasia, and chromosome abnormalities. One hundred and fifty-three women with normal pregnancy leading to live birth were selected at random to comprise the control group. All women included in this study were genetically unrelated Han Chinese women. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and allele-specific polymerase chain reaction (AS-PCR) were used to determine polymorphisms of rs10053538 and rs10515746, respectively, in all subjects. PCR products were chosen at random for sequencing.
RESULTS
No significant statistical difference was found between the distribution frequency of the GT + TT genotype and T allele on the rs10053538 locus in the URSA group or the control group (10.1% vs. 11.8%, Chi(2) = 0.205, P = 0.651; 5.1% vs. 6.5%, Chi(2) = 0.592, P = 0.441; respectively). Neither was there a significant difference between the distribution frequency of the GT + TT genotype and T allele on the rs10515746 locus in the groups (6.8% vs. 3.9%, Chi(2)1.201, P = 0.273; 3.4% vs. 2.0%, Chi(2) = 1.169, P = 0.280; respectively).
CONCLUSIONS
The present study suggested that these polymorphisms of rs10053538 or rs10515746 in the Tim-3 promoter may not be associated with URSA in Han Chinese women.
背景
复发性自然流产（RSA）指连续发生2次或更多次的妊娠丢失，病因不明的RSA称为不明原因复发性自然流产（URSA）。T细胞免疫球蛋白结构域和粘蛋白结构域（Tim）蛋白家族的一个亚型Tim-3可能是一种重要的调节分子，在URSA中起关键作用，URSA可能主要由Th1/Th2免疫失衡引发。为了解中国汉族女性URSA的病因及发病机制，我们研究了Tim-3启动子区rs10053538和rs10515746的多态性与中国汉族女性URSA风险之间的关联。
方法
148例因RSA导致死产的女性纳入URSA组。我们进行了相关检查以排除先天性生殖系统畸形、生殖系统肿瘤、内分泌失调及染色体异常。随机选取153例妊娠正常并活产的女性组成对照组。本研究纳入的所有女性均为无血缘关系的中国汉族女性。分别采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）和等位基因特异性聚合酶链反应（AS-PCR）检测所有研究对象rs10053538和rs10515746的多态性。随机选取PCR产物进行测序。
结果
URSA组与对照组rs10053538位点的GT + TT基因型和T等位基因分布频率比较，差异均无统计学意义（分别为10.1% 对11.8%，χ² = 0.205，P = 0.651；5.1% 对6.5%，χ² = 0.592，P = 0.441）。两组rs10515746位点的GT + TT基因型和T等位基因分布频率比较，差异也无统计学意义（分别为6.8% 对3.9%，χ² = 1.201，P = 0.273；3.4% 对2.0%，χ² = 1.169，P = 0.280）。
结论
本研究提示，Tim-3启动子区rs10053538或rs10515746的这些多态性可能与中国汉族女性的URSA无关。
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