E.E.A. Rafaela, INTA, Santa Fe, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina.
Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina.
Res Vet Sci. 2014 Feb;96(1):86-94. doi: 10.1016/j.rvsc.2013.10.004. Epub 2013 Oct 23.
Staphylococcus aureus is the most frequently isolated pathogen from bovine intramammary infections worldwide. Commercially available vaccines for mastitis control are composed either of S. aureus lysates or whole-cells formulated with traditional adjuvants. We recently showed the ability of a S. aureus CP5 whole-cell vaccine adjuvanted with ISCOM Matrix to increase specific antibodies production in blood and milk, improving opsonic capacity, compared with the same vaccine formulated with Al(OH)3. However, there is no information about the use of ISCOM Matrix for the formulation of bacterial lysates. The aim of this study was to characterize the innate and humoral immune responses induced by a S. aureus CP5 whole-cell or lysate vaccine, formulated with ISCOM Matrix after immunization of pregnant heifers. Both immunogens stimulated strong humoral immune responses in blood and milk, raising antibodies that increased opsonic capacity. Lysate formulation generated a higher and longer lasting antibody titer and stimulated a higher expression of regulatory and pro-inflammatory cytokines compared with the whole-cell vaccine.
金黄色葡萄球菌是全球奶牛乳腺炎最常分离的病原体。商业上用于控制乳腺炎的疫苗要么由金黄色葡萄球菌裂解物组成，要么由含有传统佐剂的全细胞组成。我们最近表明，用 ISCOM 基质佐剂的金黄色葡萄球菌 CP5 全细胞疫苗能够增加血液和牛奶中特异性抗体的产生，与用 Al(OH)3 配制的相同疫苗相比，提高调理能力。然而，关于 ISCOM 基质用于细菌裂解物配方的信息尚不清楚。本研究的目的是描述用 ISCOM 基质配制的金黄色葡萄球菌 CP5 全细胞或裂解物疫苗在怀孕小母牛免疫后的先天和体液免疫反应。两种免疫原都能在血液和牛奶中刺激强烈的体液免疫反应，产生增加调理能力的抗体。与全细胞疫苗相比，裂解物制剂产生更高和更持久的抗体滴度，并刺激更高水平的调节性和促炎细胞因子表达。
