Renna María S, Pereyra Elizabet A L, Baravalle Celina, Camussone Cecilia M, Dallard Bibiana E, Marcipar Ivan S, Calvinho Luis F
Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Santa Fe, Argentina; Instituto de Ciencias Veterinarias del Litoral (ICIVET-Litoral), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina.
FEMS Microbiol Lett. 2014 Nov;360(1):62-9. doi: 10.1111/1574-6968.12588. Epub 2014 Sep 26.
A successful Staphylococcus aureus vaccine should elicit a long-term antibody response that prevents establishment of the infection. The aim of the present study was to evaluate the functional role of antibodies raised against different S. aureus CP5 vaccines in invasion to bovine mammary epithelial cells (MAC-T) and phagocytosis by bovine milk macrophages in vitro. Sera and whey from cows immunized with a whole-cell S. aureus CP5 vaccine adjuvanted with Al(OH)3 or with ISCOM Matrix, significantly reduced internalization of S. aureus in MAC-T cells without significant differences between both groups. The effect of antibodies generated by a S. aureus whole-cell and a lysate vaccine formulated with ISCOM Matrix was also evaluated. Sera and whey from both immunized groups significantly reduced S. aureus internalization in MAC-T cells without significant differences between both groups. Whey antibodies against whole-cell and lysate vaccines were also able to inhibit internalization in MAC-T cells of a heterologous S. aureus strain. In addition, sera from animals vaccinated with S. aureus lysate or bacterin promoted milk macrophage phagocytosis. These results provide an insight into the potential mechanisms by which these vaccines can afford protection to the mammary gland against S. aureus intramammary infection.
一种成功的金黄色葡萄球菌疫苗应引发长期抗体反应，以防止感染的发生。本研究的目的是评估针对不同金黄色葡萄球菌CP5疫苗产生的抗体在体外侵袭牛乳腺上皮细胞（MAC-T）以及被牛乳巨噬细胞吞噬过程中的功能作用。用氢氧化铝或免疫刺激复合物（ISCOM）基质佐剂的全细胞金黄色葡萄球菌CP5疫苗免疫的奶牛的血清和乳清，显著降低了MAC-T细胞中金黄色葡萄球菌的内化，两组之间无显著差异。还评估了用ISCOM基质配制的金黄色葡萄球菌全细胞疫苗和裂解物疫苗产生的抗体的效果。两个免疫组的血清和乳清均显著降低了MAC-T细胞中金黄色葡萄球菌的内化，两组之间无显著差异。针对全细胞和裂解物疫苗的乳清抗体也能够抑制异源金黄色葡萄球菌菌株在MAC-T细胞中的内化。此外，用金黄色葡萄球菌裂解物或菌苗接种的动物的血清促进了乳巨噬细胞的吞噬作用。这些结果为这些疫苗能够保护乳腺免受金黄色葡萄球菌乳房内感染的潜在机制提供了见解。
