Department of Plant Breeding and Biometry, Cornell University, 14853-1902, Ithaca, NY, USA.
Plant Cell Rep. 1992 Jan;10(12):624-8. doi: 10.1007/BF00232384.
Cauliflower (Brassica oleracea L. ssp. botrytis) protoplasts with Ogura male sterile and fertile B. oleracea cytoplasms were fused, producing plants with an array of organellar types. Plants with Ogura mitochondria were male sterile; those with B. oleracea chloroplasts were cold tolerant. In some fusions, unfused parental protoplasts were eliminated by double inactivation with iodoacetate and gamma-irradiation; in others, fused protoplasts were physically isolated by micromanipulation or by cell sorting. Double inactivation fusions produced the most plants, including many which were male sterile, female fertile, cold tolerant and diploid.
花椰菜（甘蓝亚种 Brassica oleracea L. ssp. botrytis）原生质体与 Ogura 雄性不育和可育甘蓝细胞质融合，产生了具有一系列细胞器类型的植物。具有 Ogura 线粒体的植物雄性不育；具有甘蓝叶绿体的植物耐寒。在一些融合中，未融合的亲本体原生质体通过碘乙酰胺和γ辐射的双重失活被消除；在其他融合中，融合的原生质体通过微操作或细胞分选物理分离。双重失活融合产生了最多的植物，包括许多雄性不育、雌性可育、耐寒和二倍体的植物。
