Advanced Genetic Sciences, Inc., 6701 San Pablo Avenue, 94608, Oakland, CA, USA.
Plant Cell Rep. 1987 Apr;6(2):98-101. doi: 10.1007/BF00276662.
X-irradiated protoplasts of a Brassica napus line carrying the Ogura Raphanus sativus male sterile cytoplasm were fused to protoplasts of male fertile B. napus cv. Olga. Plants were regenerated from six out of 34 randomly selected clones. In one clone, Bn(RS)26, a plant with male sterile flowers was obtained. Mitochondria of this plant are non-parental as revealed by DNA-DNA hybridization using a species specific probe. Its chloroplasts, however, derive from the fertile parent which results in loss of the sensitivity to low temperatures associated with R. sativus plastids in the male sterile parent. The novel cytoplasm of the Bn(RS)26 cybrid was transmitted through seed.
X 射线辐照甘蓝型油菜品系 Ogura 萝卜细胞质雄性不育的原生质体，与雄性可育甘蓝型油菜 cv. Olga 的原生质体融合。从 34 个随机选择的克隆中，有 6 株再生植株。在一个克隆 Bn(RS)26 中，获得了一株雄性不育花的植株。通过使用物种特异性探针的 DNA-DNA 杂交，证明该植株的线粒体是非亲本的。然而，其叶绿体来自可育亲本，导致雄性不育亲本中与萝卜质体相关的对低温不敏感的丧失。Bn(RS)26 杂种的新型细胞质通过种子传递。
