量化低温电子显微镜密度图的局部分辨率。

Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA.

1] Department of Biomedical Engineering, Yale University, New Haven, Connecticut, USA. [2] Department of Cellular and Molecular Physiology, Yale University, New Haven, Connecticut, USA.

Nat Methods. 2014 Jan;11(1):63-5. doi: 10.1038/nmeth.2727. Epub 2013 Nov 10.

We propose a definition of local resolution for three-dimensional electron cryo-microscopy (cryo-EM) density maps that uses local sinusoidal features. Our algorithm has no free parameters and is applicable to other imaging modalities, including tomography. By evaluating the local resolution of single-particle reconstructions and subtomogram averages for four example data sets, we report variable resolution across a 4- to 40-Å range.

我们提出了一种使用局部正弦特征来定义三维电子冷冻电子显微镜（cryo-EM）密度图局部分辨率的方法。我们的算法没有自由参数，可适用于包括断层扫描在内的其他成像模式。通过评估四个示例数据集的单颗粒重建和子断层平均的局部分辨率，我们报告了 4 至 40 Å 范围内的可变分辨率。