用海洋蓝细菌高分子量提取物处理的胡萝卜体细胞胚的植株形成促进。

Promotion of plantlet formation from somatic embryos of carrot treated with a high molecular weight extract from a marine cyanobacterium.

机构信息

Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, 184, Tokyo, Japan.

出版信息

Plant Cell Rep. 1992 Mar;11(2):62-5. doi: 10.1007/BF00235254.

Abstract

Somatic embryos of Daucus carota L. developed into plantlets at high frequency after addition of an extract from a marine cyanobacterium, Synechococcus sp. NKBG 042902. High molecular weight, nondialyzing fraction, separated from the extract, possessed enhanced plantlet formation promoting activity. Plantlet formation frequency was 60 % after addition of nondialysate (100 mg/l) compared to 28 % without addition. Embryos treated with the nondialysate contained five times more chlorophyll than nontreated embryos after 6 days of culture. The chlorophyll a/b ratio of 4-day old treated somatic embryos was found to be similar to that of zygotic embryos. However, the chlorophyll a/b ratio of plantlets induced from nontreated somatic embryos was variable. Nondialysate was fractionated by ultracentrifugation and an active component obtained, which gave a maximum plantlet formation frequency of 71 %, and induced rapid greening of shoots.

摘要

添加来自海洋蓝藻，Synechococcus sp. NKBG 042902 的提取物后，胡萝卜体细胞胚高频发育成小植株。从提取物中分离出的高分子量、不可透析部分具有增强的小植株形成促进活性。与不添加相比，添加非透析物（100mg/L）后小植株形成频率为 60%，而添加非透析物后为 28%。处理过的胚胎在培养 6 天后比未处理的胚胎含有多 5 倍的叶绿素。发现 4 天大的处理过的体细胞胚的叶绿素 a/b 比值与合子胚相似。然而，来自未处理的体细胞胚诱导的小植株的叶绿素 a/b 比值是可变的。非透析物通过超速离心进行分级，获得一个活性成分，其最大小植株形成频率为 71%，并诱导芽的快速变绿。