Department of Biotechnology, Tokyo University of Agriculture and Technology, Koganei, 184, Tokyo, Japan.
Plant Cell Rep. 1991 Apr;9(12):655-8. doi: 10.1007/BF00235350.
Twenty five strains of marine cyanobacteria were screened for their ability to promote carrot somatic embryogenesis. Hot water extracts prepared from 21 of these strains promoted plantlet formation. Extracts from four strains increased plantlet numbers to an average of over 3.7-fold. Dialysates and nondialysates of each of these extracts also increased plantlet formation. For extracts from filamentous cyanobacteria, Nostoc sp. and Anabaena sp., dialysate was more effective (4.2-fold increase) than nondialysate (3.0-fold increase), whereas for unicellular strains Synechococcus sp. and Xenococcus sp., nondialysate was more effective (5.2-fold increase) than the dialysate (3.2-fold increase). These cyanobacterial extracts also promoted embryolike structure formation from two-year old carrot cell cultures which were unable to produce plantlets using the usual methods. Here, we demonstrate the existence in marine cyanobacterial extracts of low and high molecular weight factors which strongly promote somatic embryogenesis in carrot cell cultures.
对 25 株海洋蓝藻进行了筛选，以评估它们促进胡萝卜体细胞胚胎发生的能力。从其中 21 株蓝藻中提取的热水提取物促进了植株的形成。4 株蓝藻的提取物使植株数量平均增加了 3.7 倍以上。这些提取物的透析物和非透析物都能促进植株的形成。对于丝状蓝藻 Nostoc sp. 和 Anabaena sp. 的提取物，透析物（增加 4.2 倍）比非透析物（增加 3.0 倍）更有效，而对于单细胞菌株 Synechococcus sp. 和 Xenococcus sp. ，非透析物（增加 5.2 倍）比透析物（增加 3.2 倍）更有效。这些蓝藻提取物还促进了来自两年生胡萝卜细胞培养物的胚胎样结构的形成，而这些细胞培养物使用常规方法无法产生植株。在这里，我们证明了海洋蓝藻提取物中存在低分子量和高分子量的因子，这些因子能强烈促进胡萝卜细胞培养物的体细胞胚胎发生。
