Department of Cardiac Surgery, Medical University of Vienna, Vienna A-1090, Austria.
Int J Mol Sci. 2013 Nov 7;14(11):22042-51. doi: 10.3390/ijms141122042.
The effects of cold storage using Custodiol® (Histidine-Tryptophan-Ketoglutarate, HTK) or isotonic saline solution on mitochondrial function in hearts (left and rights ventricles) and various blood vessels of pigs were investigated. Hearts, saphenous veins, internal-mammary-arteries and aortas of male landrace pigs were harvested and exposed to cold ischemia in either saline or Custodiol-HTK solution. Mitochondrial function was measured in situ in permeabilized fibers by high-resolution respirometry. Mitochondrial respiratory capacities (maximal respiration rates) were similar in the right and left ventricle in controls and after 14 h of cold storage were significantly better preserved in Custodiol-HTK than in saline solution. Mitochondrial respiration rates in various blood vessels including aorta, arteries and veins were less than 5% of myocardium rates. In contrast to the pig heart, in some blood vessels, like veins, mitochondrial function remained stable even after 24 h of cold ischemia. HTK-Custodiol protection of mitochondrial function after prolonged cold ischemia was observed in the myocardium but not in blood vessels. HTK-Custodiol solution thus offers significant protection of myocardial mitochondria against cold ischemic injury and can be used as efficient preservation solution in organ transplantation but probably has no benefit for blood vessels preservation. Analysis of mitochondrial function can be used as a valuable approach for the assessment of cold ischemic injury in various tissues including pig heart and various blood vessels.
研究了使用 Custodiol®（组氨酸-色氨酸-酮戊二酸，HTK）或等渗盐水溶液冷藏对猪心脏（左心室和右心室）和各种血管中线粒体功能的影响。收获雄性长白猪的心脏、大隐静脉、内乳动脉和主动脉，并在盐溶液或 Custodiol-HTK 溶液中暴露于冷缺血状态。通过高分辨率呼吸测量法在渗透纤维中原位测量线粒体功能。在对照和冷储存 14 小时后，右心室和左心室的线粒体呼吸能力（最大呼吸率）相似，Custodiol-HTK 中的线粒体呼吸率明显优于盐溶液。包括主动脉、动脉和静脉在内的各种血管的线粒体呼吸率低于心肌的 5%。与猪心脏不同，在一些血管中，如静脉，即使在 24 小时冷缺血后，线粒体功能仍保持稳定。在心肌中观察到 HTK-Custodiol 在长时间冷缺血后对线粒体功能的保护，但在血管中则没有。HTK-Custodiol 溶液可有效保护心肌线粒体免受冷缺血损伤，可作为器官移植中的有效保存溶液，但可能对血管保存没有益处。线粒体功能分析可作为评估包括猪心脏和各种血管在内的各种组织冷缺血损伤的有价值方法。
