Monsanto Company, 700 Chesterfield Village Parkway, 63017, Chesterfield, MO, USA.
Plant Cell Rep. 1991 Apr;9(12):699-702. doi: 10.1007/BF00235361.
Incorporating 10 to 100 μM AgNO3 into Phytagel(™) (0.2%) solidified N6 medium containing 1 mg/L 2,4-D, 100 mg/L casamino acids and 25 mM praline (N6 1-100-25) promoted type II callus production from cultured Zea mays L. immature embryos of FRB73, B73 X A188 and a proprietary B73 BC6 genotype. Under these conditions, approximately 15, 80 and 80% of the respective FRB73, B73 X A188 and B73 BC6 explants produced type II calli after 2 to 3 weeks incubation in the dark at 28 C. In the absence of AgNO3, the type II culture response from B73BC6 immature embryos was 25% on N6 1 100-25 solidified with Phytagel(™) (0.2%) as compared to 0% for that solidified with 0.8% agar. Duncan's medium was tested using 10 to 100 μm AgNO3 and generally promoted type I callus initiation, although up to 6% of the explants produced type II cultures in the presence of 0.2% Phytagel(™). Ethylene emanation rates of up to 370 and 115 nL g-1 h-1 were detected from B73 X A188 immature embryos and calli, respectively, cultured on N6 1-100-25.
在含有 1 mg/L 2,4-D、100 mg/L 水解酪蛋白和 25 mM 脯氨酸的 0.2% Phytagel(™) 固化 N6 培养基中加入 10 至 100 μM 的 AgNO3，可促进 FRB73、B73 X A188 和专有的 B73 BC6 基因型的玉米未成熟胚培养物产生 II 型愈伤组织。在这些条件下，大约 15%、80%和 80%的 FRB73、B73 X A188 和 B73 BC6 外植体在黑暗中于 28°C 下孵育 2 至 3 周后产生 II 型愈伤组织。在没有 AgNO3 的情况下，B73BC6 未成熟胚的 II 型培养物响应在 0.2% Phytagel(™) 固化的 N6 1 100-25 上为 25%，而在 0.8%琼脂固化的 N6 1 100-25 上为 0%。测试了含有 10 至 100 μM 的 AgNO3 的 Duncan 培养基，它通常促进 I 型愈伤组织起始，尽管在存在 0.2% Phytagel(™) 的情况下，多达 6%的外植体产生了 II 型培养物。在培养于 N6 1-100-25 的 B73 X A188 未成熟胚和愈伤组织中，分别检测到高达 370 和 115 nL g-1 h-1 的乙烯排放率。
