Anwaar Sadaf, Maqbool Qaisar, Jabeen Nyla, Nazar Mudassar, Abbas Fazal, Nawaz Bushra, Hussain Talib, Hussain Syed Z
Department of Biotechnology and Bioinformatics, International Islamic University Islamabad Islamabad, Pakistan.
Department of Biotechnology, Virtual University of PakistanLahore, Pakistan; Interdisciplinary Research Organization, University of ChakwalChakwal, Pakistan.
Front Plant Sci. 2016 Aug 31;7:1330. doi: 10.3389/fpls.2016.01330. eCollection 2016.
In this study, we have investigated the effect of copper oxide nanoparticles (CuO-NPs) on callogenesis and regeneration of Oryza sativa L (Super Basmati, Basmati 2000, Basmati 370, and Basmati 385). In this regard, CuO-NPs have been bio-synthesized via Azadirachta indica leaf extract. Scanning electron microscope (SEM) analysis depicts average particle size of 40 ± 5 nm with highly homogenous and spherical morphology. X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) have been employed to confirm the phase purity of the synthesized NPs. It is found that CuO-NPs exhibit very promising results against callus induction. It is attributed to the fact that green synthesized CuO-NPs at optimum dosage possess very supportive effects on plant growth parameters. In contrast to callogenesis, differential regeneration pattern has been observed against all of the examined O. sativa L. indigenous verities. Overall observation concludes that CuO, being one of the essential plant nutrients, has greatly tailored the nutritive properties at nano-scale.
在本研究中，我们研究了氧化铜纳米颗粒（CuO-NPs）对水稻（超级巴斯马蒂、巴斯马蒂2000、巴斯马蒂370和巴斯马蒂385）愈伤组织形成和再生的影响。在这方面，CuO-NPs已通过印楝叶提取物进行生物合成。扫描电子显微镜（SEM）分析显示平均粒径为40±5nm，具有高度均匀的球形形态。X射线衍射（XRD）和傅里叶变换红外光谱（FTIR）已被用于确认合成纳米颗粒的相纯度。发现CuO-NPs在愈伤组织诱导方面表现出非常有前景的结果。这归因于以下事实：在最佳剂量下绿色合成的CuO-NPs对植物生长参数具有非常有利的影响。与愈伤组织形成相反，在所有检测的水稻本地品种中均观察到不同的再生模式。总体观察得出结论，铜作为植物必需营养素之一，在纳米尺度上极大地调整了营养特性。
