Department of Experimental Virology, Institute of Hematology and Blood Transfusion (IHBT), Prague, Czech Republic.
J Med Virol. 2014 Sep;86(9):1560-8. doi: 10.1002/jmv.23841. Epub 2013 Nov 8.
JC and BK polyomaviruses (JCV and BKV) infect humans and can cause severe illnesses in immunocompromised patients. Merkel cell polyomavirus (MCPyV) can be found in skin carcinomas. In this study, we assessed the occurrence of serum antibodies against MCPyV, BKV, and JCV polyomaviruses in a healthy population of the Czech Republic. Serum samples from 991 healthy individuals (age range: 6-64 years) were examined by enzyme-linked immunoassay (ELISA) using virus-like particles (VLPs) based on the major VP1 capsid proteins of these viruses. Overall, serum antibodies against MCPyV, JCV, and BKV were found in 63%, 57%, and 69%, respectively, of this population. For all three viruses, these rates were associated with age; the occurrence of antibodies against MCPyV and JCV was highest for those older than 59 years, while the occurrence of antibodies against BKV was highest in those aged 10-19 years and 20-29 years. This is the first large study to determine the seroprevalence rates for BKV, JCV, and MCPyV polyomaviruses in the general Czech Republic population.
JC 和 BK 多瘤病毒（JCV 和 BKV）感染人类，可导致免疫功能低下的患者发生严重疾病。 Merkel 细胞多瘤病毒（MCPyV）可存在于皮肤癌中。在这项研究中，我们评估了捷克共和国健康人群中血清抗 MCPyV、BKV 和 JCV 多瘤病毒抗体的发生情况。采用酶联免疫吸附试验（ELISA），使用基于这些病毒主要 VP1 衣壳蛋白的病毒样颗粒（VLPs），对 991 名健康个体（年龄范围：6-64 岁）的血清样本进行了检测。总体而言，该人群中分别有 63%、57%和 69%的个体血清中存在针对 MCPyV、JCV 和 BKV 的抗体。对于所有三种病毒，这些比率均与年龄相关；针对 MCPyV 和 JCV 的抗体发生率在 59 岁以上的人群中最高，而针对 BKV 的抗体发生率在 10-19 岁和 20-29 岁的人群中最高。这是首次在捷克共和国普通人群中确定 BKV、JCV 和 MCPyV 多瘤病毒血清流行率的大型研究。
