用N - 乙基马来酰亚胺处理后，大鼠甲胎蛋白对雌激素的亲和力出现惊人的增加。

A surprising increase in the affinity of estrogen for rat alpha-fetoprotein after it is treated with N-ethylmaleimide.

作者信息

Baker M E

出版信息

Biochem Int. 1986 Feb;12(2):331-40.

Abstract

Incubation of rat alpha-fetoprotein (AFP) with mM concentrations of N-ethylmaleimide (MalNEt) at 22 degrees, pH7.8 for several hours yields a form of AFP with an equilibrium dissociation constant of 0.1 nM for estrone. This affinity for estrone is about 10 fold higher than that for untreated rat AFP. MalNEt-treated AFP still binds inhibitors and substrates of serine proteases suggesting that the binding of these compounds to AFP does not involve an interaction with a thiol group. MalNEt-treated AFP may be useful for studying the mechanism of estrogen action in vitro and in vivo, estrogen-protein interactions, and the functioning of AFP.

摘要

在22摄氏度、pH7.8条件下，将大鼠甲胎蛋白（AFP）与毫摩尔浓度的N - 乙基马来酰亚胺（MalNEt）孵育数小时，可产生一种对雌酮的平衡解离常数为0.1纳摩尔的AFP形式。这种对雌酮的亲和力比未处理的大鼠AFP高约10倍。经MalNEt处理的AFP仍能结合丝氨酸蛋白酶的抑制剂和底物，这表明这些化合物与AFP的结合不涉及与巯基的相互作用。经MalNEt处理的AFP可能有助于研究雌激素在体外和体内的作用机制、雌激素 - 蛋白质相互作用以及AFP的功能。