Terent'ev A A, Moldogazieva N T, Tatarinov Iu S
Biull Eksp Biol Med. 1992 Jun;113(6):588-90.
Alpha-fetoproteins (AFP) from amniotic fluid of mouse and rat demonstrate high affinity and specificity during their binding with immobilized diethylstilbestrol, which allows to isolate these two proteins by one step using the method of affinity chromatography on Sepharose with immobilized diethylstilbestrol. Meanwhile the yield of mouse AFP was 42%, and rat AFP--75%. The preliminary incubation of the amniotic fluid of rat and mouse with free estradiol results in abrupt fall of AFP outcome, which may testify to the binding of estradiol and diethylstilbestrol by the same receptor sites on AFP molecule.
来自小鼠和大鼠羊水的甲胎蛋白（AFP）在与固定化己烯雌酚结合时表现出高亲和力和特异性，这使得可以使用固定化己烯雌酚的琼脂糖亲和色谱法一步分离这两种蛋白质。同时，小鼠AFP的产率为42%，大鼠AFP为75%。用游离雌二醇对大鼠和小鼠羊水进行预孵育会导致AFP产量急剧下降，这可能证明雌二醇和己烯雌酚在AFP分子上的相同受体位点结合。
