Lechleiter J, Wells M, Gruener R
Biochim Biophys Acta. 1986 Apr 25;856(3):640-5. doi: 10.1016/0005-2736(86)90159-8.
The single-channel recording technique was used to investigate the role of membrane lipids in the action of general anesthetics on ion channels. We examined the effects of halothane on acetylcholine receptor channels in Xenopus laevis myocytes in which the plasma membrane cholesterol level had been changed by pretreatment with cholesterol-rich or cholesterol-free liposomes. We found that the alteration in acetylcholine receptor channel kinetics, elicited in the presence of clinically-relevant concentrations of halothane, is attenuated when membrane cholesterol is increased and enhanced when membrane cholesterol concentration is decreased. These findings support the idea that general anesthetics interact with synaptic receptor channels indirectly through the lipid domains in which these synaptic proteins are embedded.
采用单通道记录技术来研究膜脂在全身麻醉药对离子通道作用中的角色。我们检测了氟烷对非洲爪蟾卵母细胞中乙酰胆碱受体通道的影响，在这些细胞中，质膜胆固醇水平通过用富含胆固醇或不含胆固醇的脂质体预处理而发生了改变。我们发现，在临床相关浓度的氟烷存在时引发的乙酰胆碱受体通道动力学改变，在膜胆固醇增加时减弱，而在膜胆固醇浓度降低时增强。这些发现支持了全身麻醉药通过这些突触蛋白所嵌入的脂质结构域与突触受体通道间接相互作用的观点。
